Journal: Scientific reports

Article Title: Action inflexibility and compulsive-like behavior accompany neurobiological alterations in the anterior orbitofrontal cortex and associated striatal nuclei.

doi: 10.1038/s41598-024-84369-1

Figure Lengend Snippet: Fig. 4. Breeding mice for poor action flexibility and compulsive-like behavior results in changes in inhibitory synapse-associated protein levels in the VLO. (a) Coronal atlas image depicting the neuroanatomical location of the aMO and aVLO. (b) EB mice had higher levels of GAD67 in the aVLO. (c) GAD67 levels in aMO. did not differ between groups. (d) Meanwhile, in the aVLO, SLITRK3 was lower. (e) SLITRK3 levels did not differ in the aMO. (f) Coronal atlas image depicting the neuroanatomical location of the pMO and pVLO. (g) GAD67 levels were higher in the pVLO of EB mice and (h) in the pMO. (i) SLITRK3 levels did not differ between groups in the pVLO or (j) pMO. Ctrl refers to same-strain age-matched mice. Data shown as fold- change from control mean + SEM with circles representing individual mice. Ctrl n = 9–10, EB n = 9–11, with representative immunofluorescent images inset. Scale bar = 50 μm. *p ≤ 0.05, **p < 0.01.

Article Snippet: Primary antibodies were GAD67 (Cell Signaling 413182; 1:5,000), SLITRK3 (Sigma SAB3500965; 1:250), SAPAP3 (Invitrogen PA520465; 1:5,000), striatin (Novus NB110-74571; 1:250), and c-fos (Abcam 190289; 1:1,000).

Techniques: Control

Journal: bioRxiv

Article Title: Anatomical and functional organization of the interpeduncular nucleus in larval zebrafish

doi: 10.1101/2024.10.09.617353

Figure Lengend Snippet: A) Position of the IPN with in the larval zebra fish brain(MapZBrain Atlas); bottom right, an horizontal view of a triple transgenicfish Tg(16715:Gal4); Tg(UAS:GCaMP6s); Tg(elavl3:H2B-mCherry) in which GCaMP6s is expressed in the habenular neurons (cyan) and mCherry is expressed pan-neuronally (red). B) Distribution of somas around the IPN region. A central dense cluster of somas (core) separates the dIPN and the vIPN; a wall of somas delimitates anteriorly the dIPN. C) A horizontal confocal section of the dIPN (left) and vIPN (right) stained with anti-GAD67 (orange) and DAPI (purple). Hb axons (green) are labeled by Tg(16715:Gal4); Tg(UAS:GCaMP6s). D) Overview of the skeletons from EM reconstructions, separated by location of the cell somata (habenula, IPN core, dorsal hindbrain regions). E) Provenance of the cells reconstructed in this paper. F) composition of the IPN neuropil by cell provenance.

Article Snippet: The brains were incubated with a rabbit anti-GAD67 antibody (PA5-21397, Invitrogen) in the blocking solution (1:300) at 4°C for 3 days, and then with an anti-rabbit IgG antibody conjugated with Alexa Fluor 568 (ab175470, Abcam) in the blocking solution (1:500) at 4 °C for 3 days.

Techniques: Staining, Labeling